Imaging Protein Statistical Substate Occupancy in a Spectrum-Function Phase Space


TitleImaging Protein Statistical Substate Occupancy in a Spectrum-Function Phase Space
Publication TypeJournal Article
Year of Publication2010
AuthorsDeWitt, W, Chu, K
JournalPhysical Review Letters
Volume105
Issue9
Date Published8/2010
ISSN0031-9007
Abstract

Hemeprotein ligand rebinding studies reveal varying IR absorbance and rebinding functions across a cryogenic ensemble. Since IR-active vibrations and rebinding barriers couple to structural coordinates, spectral and functional heterogeneity arise from conformational heterogeneity. Modeling rebinding data as a spectrally resolved superposition of first-order rate processes and employing maximum entropy regularization, protein heterogeneity is imaged as an ensemble occupancy of a spectrum-function phase space. Results from myoglobin rebinding carbon monoxide are discussed.

DOI10.1103/PhysRevLett.105.098101
Short TitlePhys. Rev. Lett.
Status: 
Published
Attributale Grant: 
IRWE
Grant Year: 
Post_Grant