Plant assimilation of phosphorus from an insoluble organic form is improved by addition of an organic anion producing Pseudomonas sp.


TitlePlant assimilation of phosphorus from an insoluble organic form is improved by addition of an organic anion producing Pseudomonas sp.
Publication TypeJournal Article
Year of Publication2014
AuthorsGiles, CD, Hsu, P-C (L), Richardson, AE, Hurst, MRH, Hill, JE
JournalSoil Biology and Biochemistry
Volume68
Pagination263 - 269
Date Published01/2014
ISSN00380717
Abstract

Improved crop utilization of native soil phosphorus (P) could reduce agricultural demand for non-renewable mineral phosphates and minimize surface water pollution. Myo-inositol hexakisphosphate (IHP) is the major organic P form in many soils, but the conversion of IHP to plant-available orthophosphate is restricted by precipitation and sorption reactions, which limit the solubility of IHP and activity of phytase enzymes. Organic anion (OA) production by Pseudomonas spp. was expected to improve the bioavailability of P in insoluble calcium-IHP (CaIHP) to Nicotiana tabacum (tobacco) plants. Bacterial production of OAs at concentrations greater than 40 mM resulted in a ∼2-unit decrease in media pH and solubilization of CaIHP salts in agar media. Plant inoculation with an OA-producing and phytate-mineralizing soil isolate (Pseudomonas sp. CCAR59) improved shoot P accumulation by wild-type (WT) and citrate-exuding (CIT) tobacco plant-lines grown on CaIHP by six- and ten-fold, respectively. Furthermore, a plant-line that exuded phytase in excess of wild type levels (PHY) assimilated three- to four-fold more P from CaIHP (0.64% shoot P) regardless of inoculation with Pseudomonas sp. CCAR59, likely due to the presence of intrinsic plant OAs and phytases. The targeted production of phytase and OAs in the rhizosphere – whether from microbial or plant sources – appears to be a promising approach for liberating orthophosphate from insoluble IHP in soils.

URLhttp://ac.els-cdn.com/S0038071713003271/1-s2.0-S0038071713003271-main.pdf?_tid=7bb8554a-c018-11e3-88c4-00000aacb360&acdnat=1397069893_d67f73f609ccad8b172d8c21cc103970
DOI10.1016/j.soilbio.2013.09.026
Short TitleSoil Biology and Biochemistry
Status: 
Published
Attributale Grant: 
RACC
Grant Year: 
Year3